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Combinatorial linear peptide libraries
X
8
to X
15
with defined
positions are highly complex pools of peptide ligands. They can be used for the rapid and unambiguous determination
of the amino acid residues responsible for binding to MHC class I and II molecules. The side chains of the amino acids
required for the T-cell recognition of the peptide/MHC complex can be determined in a chromium release assay.
Our results provide a basis, independent of the analysis of natural ligand pools
, for the
development of new assays and immunotherapeutics, as well as for the understanding of protein/peptide interactions.
In addition, cyclopeptide libraries have been developed and applied to inhibit protein/ligand interaction.
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1. S. Stevanovic, K.-H. Wiesmüller, J. Metzger, A. G. Beck-Sickinger, and G. Jung (1993).
Natural and Synthetic Peptide Pools: Characterization by Sequencing and Electrospray Mass Spectrometry,
Bioorg. Med. Chem. Lett. 1993, 3, 431-436.
2. K.-H. Wiesmüller, M. Brich, G. Jung, K. Sparbier, and P. Walden (1995).
Peptide Binding to MHC Class I Molecules Analyzed by Confocal Microscopy,
Eur. J. Cell Biol. 66, 389-393.
3. K. Udaka, K.-H. Wiesmüller, S. Kienle, G. Jung, and P. Walden (1995).
Decrypting the Structure of Major Histocompatibility Complex Class I-Restricted Cytotoxic T Lymphocyte Epitopes
with Complex Peptide Libraries,
J.Exp.Med. 181, 2097-2108.
4. S. Uebel, T.H. Meyer, W. Kraas, S. Kienle, G. Jung, K.-H. Wiesmüller and R. Tampe (1995).
Requirements for Peptide Binding to the Human Transporter Associated with Antigen Processing Revealed by Peptide
Scans and Complex Peptide Libraries,
J. Biol. Chem. 31, 19512-18516.
5. K. Udaka, K.-H. Wiesmüller, S. Kienle, G. Jung, and P. Walden (1995).
Tolerance to Amino Acid Variations in Peptides Binding to the Major Histocompatibility Complex Class I Protein
H-2K
b
,
J. Biol. Chem. 270, 24130-24134.
6. P. Walden, K.-H. Wiesmüller, and G. Jung (1995)
Elucidation of T Cell Epitopes: A Synthetic Approach with Random Peptide Libraries,
Biochem. Soc. Trans. 23, 678-681.
7. B.R. Gundlach, K.-H. Wiesmüller, T. Junt, S. Kienle, G. Jung, and P. Walden
(1996)
Specificity and Degeneracy of Minor Histocompatibility Antigen-Specific
MHC-Restricted CTL
,
J. Immunol. 156, 3645-3651.
8. B.R. Gundlach, K.-H. Wiesmⁿller, T. Junt, S. Kienle, G. Jung, and P. Walden (1996)
Determination of T Cell Epitopes with Random Peptide Libraries
,
J. Immunol. Methods 192, 149-155.
9. L. Pridzun, K.-H. Wiesmⁿller, S. Kienle, G. Jung, and P. Walden (1996)
Amino Acid Preferences the Octapeptide Subunit of the Major Histocompatibility
Complex Class I Heterotrimer H-2Ld
,
Eur. J. Biochem. 263, 249-253.
10. B. Fleckenstein, H. Kalbacher, C.P. Muller, D. Stoll, T. Halder, G. Jung, and K.-H.
Wiesmⁿller (1996)
New Ligands Binding to the Human Leukocyte Antigen Class II Molecule DRB1 *0101
Based on the Activity Pattern of an Undecapeptide Library
,
Eur. J. Biochem., 240, 71-77.
11. K. Udaka, K.-H. Wiesmⁿller, S. Kienle, G. Jung, and P. Walden (1996)
Self-MHC-Restricted Peptides Recognized by an Alloreactive T Lymphocyte Clone
J. Immunol. 157, 670-678.
12. G. Jung (ed.) (1996)
Combinatorial Peptide and Nonpeptide Libraries (566 pages, 214 figures, 73 tables)
Verlag Chemie, Weinheim.
13. K. Udaka, K.-H. Wiesmüller, S. Kienle, S. Feiertag, G. Jung, and P. Walden (1996)
Random Peptide Libraries as Tools in Basic and Applied Immunology,
in: G. Jung (ed.), Combinatorial Peptide and Nonpeptide Libraries, pp. 349-362,
Verlag Chemie, Weinheim.
14. R. Brock, K.-H. Wiesmüller, G. Jung, and P. Walden (1996)
Molecular Basis for the Recognition of Two Structurally Different Major
Histocompatibility Complex/Peptide Complexes by a Single T Cell Receptor,
Proc. Natl. Acad. Sci. USA 93, 13108-13113
cojinfo@uni-tuebingen.de(cojinfo@uni-tuebingen.de) - July 23, 1997